Journal: Molecular Metabolism

Article Title: Adipose PTEN regulates adult adipose tissue homeostasis and redistribution via a PTEN-leptin-sympathetic loop

doi: 10.1016/j.molmet.2019.09.008

Figure Lengend Snippet: Hypothalamic leptin receptor knockdown attenuates the adipose redistribution-associated with unilateral iWAT injection of Rec2-Cre in male PTEN flox mice. (A) Experimental design. (B) Hypothalamic Lepr expression. (C) iWAT weight at sacrifice. (D) Ratio of Rec2-treated iWAT to the untreated contralateral iWAT. (E) Relative mRNA expression of leptin in iWAT. (F) Ratio of leptin mRNA between Rec2-treated iWAT and untreated contralateral iWAT. Data are means ± SEM. n = 6–7 per group for body weight and tissue weight data. n = 4–6 for qPCR data. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: To select the most effective microRNA, the miR-Lepr expressing plasmids were co-transfected with a LepR expression plasmid (Lepr, MR226855, ORIGENE) to HEK293T cells.

Techniques: Injection, Expressing

Journal: Heliyon

Article Title: Improved leptin sensitivity and increased soluble leptin receptor concentrations may underlie the additive effects of combining PYY [ [1] , [2] , [3] , [4] , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] ] and exendin-4 on body weight lowering in diet-induced obese mice

doi: 10.1016/j.heliyon.2024.e32009

Figure Lengend Snippet: PYY(3 – 36) and Ex4 in combination increases leptin sensitivity and increases the concentration of circulating soluble leptin receptor. A-D) Accumulated food intake (kcal) and body weight change (g) 4h (A, B) and 20h (C, D) after leptin injection(5 mg/kg) at treatment day 10.E) Plasma leptin concentration (pM) at day 15, F: Plasma concentration of soluble leptin receptor (SLR) at day 15, and G: Hepatic Lepra expression at day 15. Data are expressed as means ± SEM, n = 12. Statistical significance was assessed by one-way ANOVA (E) or by two-way ANOVA followed (F and G). In both cases, ANOVA test's were followed Tukey's multiple comparisons test. In case of A-D, test of significance was restricted to comparing within group effects (vehicle and leptin treatment). *p < 0.05, **p < 0.01, and ***p < 0.001.

Article Snippet: Soluble leptin receptor was analysed by a mouse leptin receptor picokine ELISA kit (BOSTER biological technology, Cat # EK0440).

Techniques: Concentration Assay, Injection, Clinical Proteomics, Expressing

Journal: PLoS ONE

Article Title: Glucose-Induced O 2 Consumption Activates Hypoxia Inducible Factors 1 and 2 in Rat Insulin-Secreting Pancreatic Beta-Cells

doi: 10.1371/journal.pone.0029807

Figure Lengend Snippet: Diabetic Lepr db/db mice ( db/db ) and non-diabetic Lepr db/+ mice ( db/+ ) were killed and their pancreas, spleen and duodenal loop were dissected and fixed in paraformaldehyde solution (4%) overnight at 4°C. A–D, HIF1α expression in 10 week-old male db/db mice (n = 2; blood glucose = 25.4 and 24.1 mmol/l; body weight = 45 and 44 g) and non-diabetic db/+ mice (n = 2; blood glucose = 6.3 and 7.9 mmol/l, n = 2; body weight = 23 and 26 g). E–H, corresponding images with merged HIF1α (red) and Hoechst 33342 (blue) nuclear staining. White arrows, HIF1α-positive islet cell nuclei (purple on merged images). Bar scale = 50 µm. are representative for two animals of each kind. I–T, pimonidazole protein-adducts in 26 week-old male and female db/db mice (M–T) (n = 3; blood glucose = 25.3±0.9 mmol/l; body weight = 54±3 g) and non-diabetic db/+ mice (I–L) (n = 3; blood glucose = 6.6±0.3 mmol/l; body weight = 28±0.9 g) injected with pimonidazole (60 mg/kg) 24 h before death (brown 3,3′-diaminobenzidine precipitate). Black arrows, cells with higher pimonidazole-protein adduct intensity. Bar scale = 50 µm. are representative for two animals of each kind. U–X, immunodetection of HIF1α and pimonidazole-protein adducts in the duodenal mucosa of db/+ or db/db mice.

Article Snippet: Lepr db/db and Lepr db/+ mice on a C57BL/KSJ background were from Janvier (Le Genest-Saint-Isle, France) or from the animal facility of the Garvan Institute.

Techniques: Expressing, Staining, Injection, Immunodetection